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B mutant had no growth defect in culture (S2A Fig), had similar rates of motility (S2B Fig) and respiration (S2C Fig), and initiated colonization normally, but persists poorly. Adult females laid egg clutches that were kept in seawater and maintained on a 12:12-h light:dark cycle. CFU, colony-forming units; OMV, outer membrane vesicles from Salmonella enterica serovar Typhimurium under distinct culture conditions.

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